Leica Biosystems CM1510-S - Manual (Page 27)

27 Leica CM1510 S – Cryostat
6.2.2 Specimen orientation
• Loosen screw (4).
• Use lever (5) to orient the specimen on the ball socket.
• Retighten screw (4).
6.3 Inserting the knife or blade into the knife holder
All components of the cryostat as well as the knife or blade and
the tools for specimen preparation should be precooled in the
cryostat before starting sectioning.
• Insert the precooled knife or blade in the knife holder and clamp.
For further details on how to handle the knife holder, please refer to the
separate instruction manual for your knife holder.
• Lock handwheel with handle in upper posi-
tion.
• If the knife holder and a knife are in place,
cover the knife edge with the knife guard.
• Loosen clamping screw (1) on the speci-
men head.
• Insert the shaft of the specimen disc (3)
with the frozen specimen into the opening
(2) of the specimen head.
Make sure that the shaft of the specimen disc
is fully inserted. The entire rear surface of the
specimen disc must fit closely against the
specimen head.
• Retighten clamping screw (1).
Fig. 22
1
3
2
4 5
6.2.1 Fixing the specimen discs in the specimen head
6. Daily work with the instrument