Leica Biosystems CM1510-S - Manual (Page 28)

28 Instruction manual V 1.4 – 04/2010
6. Daily work with the instrument
6.4 Sectioning / trimming
For all settings on knife holder and anti-roll guide, please refer
to the separate instruction manual for your knife holder.
Be careful when handling microtome knives and disposable
blades. The cutting edge is extremely sharp and can cause se-
vere injury!
• Remove the knife guard from the cutting edge.
• Remove anti-roll guide from knife.
• Insert precooled knife / blade into knife holder.
• Select appropriate clearance angle on knife holder. Settings be-
tween 4° - 6° (knife holder CN) and 1° to 2° (knife holder CE) are ap-
propriate for most applications.
• Unlock the handwheel.
• Rotate the handwheel cautiously and check if the specimen comes
in contact with the cutting edge of the knife.
• Adjust knife / knife holder relative to the specimen.
• To trim the specimen, select a section thickness of approximately 30
µm with the section thickness selection knob on the front of the mi-
crotome.
• Trim the specimen to the desired sectioning plane, gradually de-
creasing the section thickness.
• Select the desired section thickness and start sectioning.
• During sectioning and for picking up the sections, place the anti-roll
guide on the knife and adjust relative to the cutting edge.
Readjust the anti-roll guide when necessary.