Leica Biosystems CM1510-S - Manual (Page 29)

29 Leica CM1510 S – Cryostat
6.4.1 Moving the specimen with the coarse / precision feed
The specimen movement towards the cutting edge must be ob-
served carefully to avoid that the specimen collides with the
cutting edge which can cause severe damage both to the knife
and the specimen!
• Press the coarse feed button for fast or slow specimen movement
towards the cutting edge.
6.4.2 Section thickness setting
To select the section thickness, rotate knob (1) to a setting between 1
and 60 µm:
from 0 - 10 µm in 1-µm steps,
from 10 - 20 µm in 2-µm steps,
from 20 - 60 µm in 5-µm steps.
The graduation scale on the microtome reflects the selected section
thickness.
• Select the desired section thickness via the rotary knob on the mi-
crotome.
Start sectioning at approx. 20 µm.
• Decrease section thickness continually down to the appropriate value.
After changing from one section thickness to another, the first two or three sections should be
rejected.
• When sectioning, turn the handwheel clockwise at a constant speed.
6. Daily work with the instrument
Fig. 23
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Fig. 24