Leica Biosystems CM1510-S - Manual (Page 42)

42 Instruction manual V 1.4 – 04/2010
Spray disinfection with Leica Cryofect
1. Select a temperature of -20 °C or higher.
2. Remove knife or blade from the knife holder.
3. Remove all specimen material, microscope slides and tools from the
cryochamber.
4. Remove section waste from the cryochamber.
5. Allow the cryochamber to reach the previously selected tempera-
ture; once that temperature is reached, either
5a.spray the disinfectant evenly on the contaminated surfaces - the
surfaces should be covered with an even layer - or
5b.soak a cloth with disinfectant and apply it on the contaminated sur-
faces.
6. In either case, allow at least 15 minute contact time.
7. Wipe up with a cloth.
8. Dispose of cloth in compliance with the applicable waste disposable
regulations of your laboratory.
9. Set cryochamber temperature back to the originally selected value.
If increased frost buildup occurs, start a manual defrost cycle.
• Place an appropriate vessel under the drain tube on the right side of
the cabinet.
• Pull the stopper at the bottom of the cryochamber to drain any con-
densate, waste or cleaning liquid that may have collected in the
cryochamber.
• Replace the stopper.
• Dispose of waste liquid according to the applicable local waste dis-
posal regulations.
All components removed from the cold environment will collect
condensation. Therefore, all components should be dried thor-
oughly before placing them back into the cryochamber.
Do not use external heaters for drying the cryochamber. This can
cause severe damage to the cooling system!
8. Cleaning and maintenance