Leica Biosystems CM1510-S - Manual (Page 35)

35 Leica CM1510 S – Cryostat
7. Troubleshooting
Problem Cause Remedy
Sections not properly flattened
despite correct temperature and
correctly aligned anti-roll plate
Sections curl on the anti-roll
plate
Scraping noise during section-
ing and specimen return move-
ment
Rippled sections
Chatter during sectioning
- Knife/blade and/or anti-roll
plate dirty.
- Edge of anti-roll plate dam-
aged.
- Blunt knife/blade.
- Anti-roll plate does not pro-
trude far enough beyond the
cutting edge.
- Anti-roll plate protrudes too far
beyond the cutting edge;
scraping against the specimen.
- Knife/blade damaged.
- Edge of anti-roll plate dam-
aged.
- Specimen insufficiently frozen
onto the specimen disc.
- Specimen disc not clamped
tightly.
- Specimen holder ball joint not
clamped.
- Knife/blade not clamped tight-
ly enough.
- Section thickness too thick -
specimen has detached from
the specimen disc.
- Very hard, inhomogeneous
specimen.
- Blunt knife/blade.
- Cutting geometry (profile) of
knife used not suitable.
- Incorrect clearance angle.-
- Clean with dry cloth or brush.
- Replace plate.
- Use different part of the cut-
ting edge or replace.
- Readjust correctly.
- Readjust correctly.
- Use different part of the cut-
ting edge or replace.
- Replace the plate.
- Refreeze specimen onto the
disc.
- Check disc clamping.
- Check ball joint clamping.
- Check knife/blade clamping.
- Refreeze specimen onto the
disc.
- Increase section thickness;
reduce specimen surface area
if necessary.
- Use different part of the cut-
ting edge.
- Use knife with different cut-
ting geometry.
- Adjust clearance angle.