Bio-Rad Model 680 Microplate Reader Accessories User Manual
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in this memory.
The first time the instrument is turned on, or after a battery failure, the following default
information will be held in the memory.
1. Both
of Administrator and Common user security password are set to "00000".
2.
Laboratory name is "Bio-Rad Laboratories".
3.
All wavelength values of the filters are set to "---" nm.
4.
The date and time of the calendar chip are set to 1 day, 1 month, 00 year, 0 hour
and 0 minute.
5.
None of plate reading data is available.
6.
End-point protocol is chosen and the protocol number is set to #1.
7. None of Standard curve data for the storage of standard curves and graphs is
available.
8.
External printer type is set to ASCII type.
4.2.4 Limits
The Microplate Reader displays absorbance readings with absolute values as 3.500.
Out-of-range absorbance values, i.e. those with absolute values greater than 3.500, are
displayed as either "*.***" or "-*.***". For example, if the absorbance is 4.500, then the display
will read "*.***", and if the absorbance is -4.500, the display will read "-*.***".
4.2.5 Reports
The Microplate Reader can generate nine types of reports for End-point protocol: Raw data,
Absorbance, Limit, Matrix, Cutoff, Curve fit, Concentration, Difference, and TeSeE Screening
report. And four types of Kinetic protocol can be generated by the reader: Absorbance,
Kinetic Plots, Linear regression, and GALT report.
Reports of End-point Protocol
(1) Raw data report
The Raw data report is the uncorrected absorbance values (without blank
subtraction). In single-wavelength mode, the reported value is the measured
absorbance. In dual-wavelength mode, the reported value is the difference between
the uncorrected readings taken with the measurement filter and with the reference
filter.
(2) Absorbance
report
The Absorbance report is the blank-corrected absorbance values. The mean
absorbance value of all of the wells designated as assay blanks is calculated and
then subtracted from all 96 values of the raw data set to produce the Absorbance
report.
Abs = Raw – Blank mean