Bio-Rad Model 680 Microplate Reader Accessories - Manual (Page 33)

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c. Cutoff Formula setting
Selection of Formula
This menu screen selects a formula for cutoff calculation. The formula marked
with “[ ]” is the current selection. The Up and Down arrow key moves the cursor
to the desired type of the formula. The movement of the cursor also accompanies
the scroll of this screen. The Enter key seals the selection and resumes to the
next setting screen.
The supported formula types are:
1. k * CNx
2. k * CPx
3. k * COx
4. CNx / k
5. CPx / k
6. COx / k
7. k + CNx
8. k + CPx
9. k + COx
10. k*CNx + CPx
11. ( CNx + CPx ) / k
12. k1*CNx + k2*CPx
Coefficient for Cutoff Formula #1 to #11
The Numerical keys and the Decimal point key are used to
enter the coefficient "k" and gray zone values. The Up and
Down arrow key moves the cursor to the desired parameter
of the cutoff formula. The Enter key sets all values of the
cutoff formula and returns to the previous screen.
Coefficient for Cutoff Formula #12
The Numerical keys and the Decimal point key are used to
enter the coefficient "k1" and "k2" and gray zone values. The
Up and Down arrow key moves the cursor to the desired
cutoff formula. The Enter key sets the all values of the cutoff
formula and returns to the previous screen.
NOTE:
The "CNx" means the mean absorbance value of Negative
control wells, the "CPx" is the mean absorbance value of
Positive control wells and the "COx" is the mean absorbance
value of Cutoff control wells. These wells are defined on the
plate map setting. The cutoff value is calculated using the
defined formula with the defined values and coefficients.
Input coefficient
k1 = 0.01 (0.01-10)
k2 = 10.0 (0.01-10)
Gray zone: 50 %(0-99)
Input coefficient
k = 0.01 (0.01-10)
Gray zone: 50 %(0-99)
k1 * CNx + k2 * CPx
k * CNx
k * CPx
k * COx
CNx / k
CPx / k
COx / k
k + CNx
k + CPx
k + COx
k * CNx + CPx
(CNx + CPx) / k
↑ »■CNx / k
•CPx / k
•COx / k
↓ •k + CNx
Select formula
»■[k*CNx ]
•k*CPx
↓ •k*COx
↑ »■k + CPx
•k + COx
•k*CNx + CPx
↓ •(CNx + CPx)/ k
↑ »■k1*CNx + k2*CPx
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