Plate map setting procedure, A. manual mapping, Select mapping mode menu – Bio-Rad Model 680 Microplate Reader Accessories User Manual

Page 40: Manual mapping

Advertising
background image

36

4.3.11. Plate Map setting procedure

Select Mapping mode Menu

This menu screen is entered by choosing the "Mapping" in the End Point Protocol
Menu screen or the Kinetic Protocol Menu screen. It is used to set the plate format
(plate mapping). The Up and Down arrow key moves the cursor next to the desired
item of menu list. The Enter key seals the selection and changes this screen to the
next setting screen.


a. Manual Mapping

Manual Mapping

In this screen, the user can set the plate format manually. 10 well types as below
are available to set.

Well type:

The supported well types are:

Well type

Indication on LCD

1. Blank:

B

2. Sample:

X00…X99

3. Standard:

S01…S99

4. In-kit Control: Positive Control

CP0…CP9

5.

Weak Positive Control CW0…CW9

6.

Negative Control

CN0…CN9

7.

Cutoff Control

CO0…CO9

8. Calibrator

CL0…CL9

9. QC Control:

QC0…QC9

10. Empty well:

-


The key assignments of well type are:

“0/EMP” --- Empty well
“1/SMP” --- Sample well
“2/BLK” --- Blank well
“3/STD” --- Standard well
“4/CO” --- Cutoff Control well
“5/QC” ---

QC

well

“6/CAL” --- Calibrator well
“7/CP” --- Positive Control well
“8/CN” --- Negative Control well
“9/CW” --- Weak Positive Control well

Two edit modes in plate mapping:

There are two editing modes, or the well type input mode and the well number
input mode. The current input mode is displayed at the upper left corner of the
screen. In case of the well type input mode, the mode sign is “[F]”. And in case of
the well number input mode, it is “■N■”.
The cursor that encloses the editing position of well has two types of enclosures
depending on the mode. For example, “[S01]” accepts the well type keys that be
assigned to the numerical keys and “■S01■” accepts the numerical input.
The mode is easily changed with the Decimal point key.

Key functions:

The Up, Down, Right and Left arrow key moves the cursor to the desired well.
The movement of the cursor also accompanies the scroll of this screen. The Left
arrow key will return the screen to the preceding screen, or the "select Mapping
mode Menu", only if the cursor is on the column #1. At this time, the selection
screen appears (See the left figure).
The Decimal point key changes the input mode.
The Numerical keys have two functions that one is used to input the number and
another is used to select a well type.
The Enter key checks the consistency between the edited plate map and other
parameters, memorizes the plate map into the protocol and changes this screen
back to the preceding screen. If it founds any inconsistency, a warning message
will be appeared on the screen.

Select Mapping mode

»■Manual mapping

•Automatic mapping

■N■ 1 2 3 4

A│■ B■ CN1 S01 S01

B│ B CN1 S02 S02

C│ B CN2 S03 S03

[F] 5 6 7 8
A│ X11 X12 X13 X14

B│ X21 X22 X23 X24

C│ X31 X32 X33[X34]

Discard change?

1:Yes 2:No

Press 1 or 2 key

Advertising