Luminex 100 IS Version 2.3 - Manual (Page 109)

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MAP Technology Using Luminex 100 IS 2.3 Software
PN 89-00002-00-072 Rev. C 5 - 67
2. On the Acquisition Detail toolbar, click New Advanced Batch.
The Options Dialog Box opens with the General tab displayed.
See Figure 5-35.
.
Figure 5-35. Options Dialog Box—General Tab
3. Enter the Name, Description, and Operator information.
4. Edit the following information as desired:
• Sample Size - use values from 20 to 200 µL. To avoid air
uptake, we recommend that your sample well contains at least
25 µL in addition to the sample size.
• DD Gate - use values within the range of 0 to 32767.
• Timeout - use values of 0 to 400, where 0 = no timeout.
5. Click the arrow down next to 100 regions to select the desired
bead map you want to view. The available bead maps are 25, 50,
64, and 100 (default) regions. Select whether you want the Bead
Events results to be displayed as Per Bead or Total Beads. If
you select Total Beads, enter the number of total beads in the
text box.
• Select Per Bead to continue analyzing until each bead set has
met at least the minimum events as determined on the Bead
Set tab.
• Select Total Beads to continue analyzing until the selected
beads meet total beads value. Use Total Beads when you are
not using all of your selected bead sets in each well. Set the
total to desired high value.
6. Click the Bead Set tab. Select the checkboxes next to each
desired bead set for this batch. Click Select All to select all the
listed bead sets, or Deselect All to deselect all selected bead sets.