Luminex 100 IS Version 2.3 - Manual (Page 98)

Luminex 100 IS User Manual Version 2.3
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MAP Technology
5 - 56 PN 89-00002-00-072 Rev. C
2. Click Clear in the dialog box.
3. Click Yes to confirm that you want to clear the batch.
Replay a Batch You can reprocess batches through the system multiple times using
Replay Batch. Replay Batch uses the data stored in the run files from
the initial acquisition to reprocess a batch, creating a new batch
output file.
Each time you reprocess a batch using Replay Batch, the system
handles it as if it is a new batch; thus, creating a separate processed
batch entry and output file. The initial batch data and output file
always remain intact and unchanged.
You reprocess a batch using Replay Batch to:
• Run as demonstrations to see how the system processes samples
and analyzes the results.
• Test a batch using different parameters, such as setting a
different number of events to be collected or using a different
bead map or new formula for analysis, also using a different
template.The number of beads for collection must be less than or
equal to the number of previously collected in the original
sample.
If you reprocess a batch with the same template parameters in a
different template, the system obtains results identical to the original
batch. If you reprocess a batch using changed parameters, the system
may obtain different results. When you replay a batch it labels
unknown samples as Pa1, Pa2, Pa3, and so on. If you replay a batch
containing replicates, replicate averaging will not be calculated in
data analysis.
A number of variables can affect the final test results. You may also
change the standards or controls processed with the batch or multi-
batch. These variables may effect your test results:
• minimum number of events for acquisition
• formula used to analyze the MFI values
• standards or controls validation or invalidation
• type of analysis (qualitative, quantitative, acquisition only, or
maintenance)