Luminex 100 IS Version 2.3 - Manual (Page 164)

Luminex 100 IS User Manual Version 2.3
x
MAP Technology
7 - 10 PN 89-00002-00-072 Rev. C
Bead Detail
Irregularities
Use these tools to assist in diagnosing system and kit-related
problems:
• system calibrators
• system controls
• assay standards
• assay controls
• error messages
The calibration lot number
selected in setup is incorrect. Enter the correct calibration lot
number in the Update CAL
Targets dialog box.
Target values for chosen
calibration lot number are
incorrect. Enter correct target values in the
Update CAL Targets dialog
box.
The wrong wells are selected in
the Setup XY tab. Ensure that the correct wells are
selected in the Run Batch tab.
Slow or unsuccessful sample
acquisition The air pressure is out of range. See Pressurization on page 7-3.
The sample probe is clogged. Clean the sample probe. See
page 6-4.
The sample probe is not
vertically aligned. Adjust the sample probe height.
See page B-8.
Air is present in the system. Run three Prime commands,
then resume sample acquisition.
The acquisition volume is set
too high. Set the acquisition volume to at
least 25 µL less than the actual
volume in your wells. This
setting lets the analyzer acquire
sample more efficiently with
less chance of acquiring air.
The xMAP microspheres are not
fully suspended. Vortex or pipette the samples up
and down to ensure that beads
are present in the solution.
You are using photobleached
microspheres. Replace photobleached
microspheres with a fresh lot of
microspheres.
There is an insufficient number
of beads in the sample. Ensure that there are 2000-5000
beads per set per well.