Luminex 100 IS Version 2.3 - Manual (Page 161)

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MAP Technology Troubleshooting
PN 89-00002-00-072 Rev. C 7 - 7
Calibration and
Control Problems
The analyzer is misaligned with
the XYP instrument. Reposition the Luminex 100
analyzer on top of the Luminex
XYP instrument so that the
holes match up correctly. The
alignment guide should tighten
and loosen fairly easily if the
instruments are aligned
correctly. Continue to square the
instruments until the guide
loosens and tightens easily.
The sample probe is bent. Remove the sample probe from
the Luminex 100 analyzer. Roll
it on a flat surface. If it does not
roll smoothly, replace it with a
new sample probe.
Symptom Possible problem Solution
Calibration is slow or fails. The calibration microspheres
are not fully suspended. Vortex the calibration vials to
resuspend the microspheres.
Wrong calibration lot number or
target values are entered in
Update CAL Targets dialog
box. Verify that the correct lot
number and target values are
used. See page 5-46 for further
information.
The system calibrators are in the
wrong well on the plate. Verify that you placed the
calibrator into the correct well.
See page 5-46.
Not enough calibrator
microspheres added to the well. Make sure that you use four or
five drops of calibrator
microspheres to the well.
Calibrator lot is expired. Use a new bottle of calibrator
beads.
The sample probe height is
incorrect. Adjust the sample probe height.
See page B-8.
The sample probe is clogged. Clean the sample probe. See
page 6-4.