Luminex 100 IS Version 2.3 - Manual (Page 119)

x
MAP Technology Using Luminex 100 IS 2.3 Software
PN 89-00002-00-072 Rev. C 5 - 77
There are two main assay types: non-competitive (such as a “capture
sandwich”) and competitive. In a non-competitive assay, the slope of
a concentration versus Mean Fluorescent Intensity (MFI) standard
curve is a positive number. That is, low concentrations result in low
MFIs and high concentrations result in high MFIs. Conversely,
competitive assays generate a standard curve with a negative slope,
the endpoints of which are high MFI/low concentration on the left,
and low MFI/high concentrations on the right.
You may direct the system to acquire samples in replicate regardless
of batch type. For qualitative batches, replicate values are averaged
and the reported interpretation is determined from this replicate
average.
Replicates in quantitative batches are based on a standard curve that
is generated by either the “Fit of all standards” or “Mean of
replicates”. The chosen curve fit is defined by the assay developer
when defining an assay template. The default is “Fit of all
standards”. Unknown samples are calculated from the standard
curve. Replicate samples are averaged to determine the reported
quantitative result denoted as “AVG”.
The system can analyze only batches that it acquires using qualitative
or quantitative templates. It does not analyze acquisitions using Data
Collection Only or Maintenance templates.
For an overview of the Analysis window, see page 5-22.
Customize Data
Analysis Settings
You can customize how the sample data results are displayed in the
analysis graph on the Standards tab. There are two options that allow
you to customize the display. Use the Customization dialog box and
the Graph Menu (right-click menu); some items are available on
both. You can define the general features of the graph, axis settings
and increments, fonts, colors, and styles presented in the graph
representing the sample data results. You also can export the analysis
to a graphic, file, clipboard, and so on.
‹
To modify the general features of the Standards tab graph:
1. Click Data Analysis.
2. Double-click the desired batch. The Analysis window opens
displaying the Standards tab.
Note: Luminex does not rec-
ommend performing Data
Analysis while the Luminex
System is performing data
acquisition on another batch.