Luminex 100 IS Version 2.3 - Manual (Page 166)

Luminex 100 IS User Manual Version 2.3
x
MAP Technology
7 - 12 PN 89-00002-00-072 Rev. C
Symptom Possible problem Solution
Calibration microspheres
classify too high. You may be using
photobleached calibration
microspheres. Replace the microspheres with a
fresh batch. To avoid
photobleaching, protect your
microspheres from light.
xMAP microspheres hit the
lower right of the region. You may be using
photobleached xMAP
microspheres. Replace microspheres with a
fresh batch. To avoid
photobleaching, protect your
microspheres from light.
Beads appear scattered. There is air in the system. Verify sample probe height. Run
2-3 Alcohol Flush commands.
The sheath fluid is empty. Make sure there is sheath fluid
in the sheath container. Prime
the system until all air is out of
the system.
Microspheres appear as a long
diagonal line. The xMAP microspheres have
agglutinated. Add additional detergent to the
assay buffer. For example, add
.02% to 0.1% Tween-20,
Triton
® X100, or SDS.
The solvent is incompatible. View a list of incompatible
solvents at the Luminex
Technical Support website -
http://luminexcorp.custhelp.com.
If the solvent you are using is
listed, switch solvents.