Importing templates -52 – Luminex 100 IS Version 2.3 User Manual

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Luminex 100 IS User Manual Version 2.3

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MAP Technology

5 - 52

PN 89-00002-00-072 Rev. C

You can group batches together as a multi-batch. Multi-batches can
consist of any number of batches that have been setup from different
assay templates and are processed consecutively.

The assay kit manufacturer may provide templates in the kits, which
they distribute on diskette or CD. Templates typically include assay
standards, controls, and maintenance commands (such as washes or
primes to acquire along with samples). OEM manufacturers may
provide templates pre-installed with your system.

The kit manufacturer includes assay reagents in the assay kit. You
must provide information about these reagents, such as lot numbers
and concentration values for the standards and assay controls.

A batch can include samples across more than one plate. When
setting up a batch, if the number of samples exceeds the wells in one
microtiter plate, another plate appears for additional samples. The
new plate appears to the immediate right of the existing plate image
on the screen with a dark line between the adjacent columns of the
two plates.

During acquisition, the Run Batch tab displays the wells containing
the samples in the microtiter plate. Colors indicate the progress in
analyzing the samples. The following well colors indicate well-
acquisition states:

Green well:

with command number—sample not
acquired.

Yellow well:

sample currently in acquisition

Red well:

sample failed. Check the system monitor for
more information

Green background: with check mark—successfully completed

Common sample errors are due to lower number of events acquired
than established in the template.

Importing

Templates

You need to import new templates to the system only once. You
must enter lot information for the standard and control reagents as
specified in the template. This lot information is used for every batch
setup using the template until it is changed.

Templates include standards, controls, both standards and controls,
maintenance commands, and acquisition commands.

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