Luminex 100 IS Version 2.3 - Manual (Page 163)

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MAP Technology Troubleshooting
PN 89-00002-00-072 Rev. C 7 - 9
Acquisition
Problems
Not enough control
microspheres added to the well. Make sure that you use at least
four or five drops of control
microspheres to the well.
Control lot is expired. Use a new bottle of control
microspheres.
The sample probe height is
incorrect. Adjust the sample probe height.
See page 6-8.
The sample probe is clogged. Clean the sample probe. See
page 6-4.
There is air in the system. Run a prime and alcohol flush.
Possible problem with the
lasers. View the system control trend
report. Check for dramatic
changes in temperature, sheath
pressure, or voltages. If any of
these situations are evident on
the report, Contact Technical
Support.
Symptom Possible problem Solution
Acquisition fails or slows. The air pressure is out of range. See Pressurization on page 7-3.
The sample probe is not
vertically aligned Adjust the sample probe height.
See page B-8.
The sample probe is clogged. Clean the sample probe. See
page 6-4.
The sheath bottle has a leaky
seal. Make sure that the sheath bottle
lid is tightened. Remove and
replace the sheath bottle lid.
The sheath or waste lines are
not fully connected. Check to make sure all tubes are
tightly connected.
The Calibration microspheres
have expired. Replace old microspheres with a
fresh lot.