Luminex 100 IS Version 2.3 - Manual (Page 88)

Luminex 100 IS User Manual Version 2.3
x
MAP Technology
5 - 46 PN 89-00002-00-072 Rev. C
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To set the Luminex XYP instrument heater temperature:
1. Click Eject/Retract to eject the plate holder.
2. Set the Luminex XYP heater block on the plate holder.
3. Click Eject/Retract to retract the plate holder.
4. In the Temperature and Gauges area of the Run Batch tab,
click Turn ON. The light on the button turns green and the
thermometer fluid turns red as it raises to reach the target
temperature.
5. Use the up and down arrows beneath the target temperature box
to set the temperature you want the Luminex XYP instrument
heater block to maintain. The user definable heater range is 35°C
to 60°C.
6. Wait for the heater to reach your selected temperature and
stabilize before processing samples. The thermometer turns
green when the temperature is stabilized.
Calibration Procedures
Calibrator xMAP microspheres are used to normalize the settings for
the reporter channel, both classification channels, and the doublet
discriminator channel. Control xMAP microspheres are used to
verify calibration and optical integrity for the system.
Calibrate the system at least once a month and:
• Following installation
• if the system is moved.
• if a part is replaced.
• if the delta calibration temperature shown on the system
monitor (on the Diagnostics tab) is more than ±3 degrees.
• if sample acquisition is problematic.
Each step in the calibration procedure usually takes less than one
minute. You must run xMAP controls after each calibration. Once
calibrated, the calibration values remain until you calibrate again.
You can track system calibration and verification results through the
Calibration Trend Report and the System Control Trend report. If
you need target value information for Calibration or Control
microspheres, you can find the information on the Luminex website
at
http://www.luminexcorp.com . Click on the Support link then
navigate to the FAQ page on the Support page.