Luminex 100 IS Version 2.3 - Manual (Page 191)

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MAP Technology Luminex 100 IS System Setup
PN 89-00002-00-072 Rev. C B - 9
2. In a 96-well microtiter plate where overall height is no more than
0.75 inches (19 mm), place the appropriate alignment tool in the
plate:
For a standard plate with flat-bottom wells—stack two of the
larger (5.08 mm diameter) alignment discs together and place
them into the selected well.
For a filter bottom plate—stack three of the larger (5.08 mm
diameter) alignment discs together and place them into the
selected well.
For a half-volume plate with flat-bottom wells—stack two of
the smaller (3.35 mm diameter) alignment discs together and
place them into the selected well.
For a round-bottom (U-bottom) plate—stack two of the
smaller (3.35 mm diameter) alignment discs and place them into
well A1.
For a plate with conical wells—place one alignment sphere into
the selected well.
3. Select the Maintenance tab, then click Eject/Retract to eject
the plate holder. Place the 96-well microtiter plate on the
Luminex XYP instrument plate holder with position A1 in the
top left corner. Click Eject/Retract to retract the plate.
4. Use the 3/32 inch hex wrench to loosen the height adjustment
locking screw. See Figure B-9.
5. Verify that the correct location is selected with the appropriate
number of discs. The location must be the same as indicated in
the software or the location you choose. Click Sample Probe
Down to lower the sample probe.
6. Using the thumb wheel, lower the probe until it just touches the
top of the alignment discs or sphere.
7. Use a 3/32 inch hex wrench to tighten the height adjustment
locking screw.
8. Click Sample Probe Up to raise the sample probe.
9. Replace the plastic shield that covers the sample probe area.
Install the Luminex XYP
Instrument Reservoir
Use the Luminex XYP instrument reservoir for the Luminex 100
analyzer maintenance functions.
Note: Alignment discs can be
placed in any well as long as
the well is designated in the
software.