Luminex 100 IS Version 2.3 - Manual (Page 40)

Luminex 100 IS User Manual Version 2.3
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MAP Technology
4 - 2 PN 89-00002-00-072 Rev. C
Photodiodes and a photomultiplier tube receive fluorescent signals
from xMAP microspheres. The Luminex 100 analyzer digitizes the
waveforms and delivers the signals to a digital signal processor
(DSP). Proprietary algorithms function with the DSP to greatly
increase sensitivity.
xMAP Microspheres
The xMAP microspheres are highly uniform, polystyrene particles
that have been crosslinked during polymerization for physical and
thermal stability. Varying amounts of fluorochromes embedded
within each xMAP microsphere give each xMAP microsphere set an
unique fluorescent signal. To ensure the stability of this signal, it is
essential to protect the microspheres from light. Follow the product
information sheet instructions for storage procedures for xMAP
microspheres and assay kits.
Calibrator xMAP microspheres are used to normalize the settings for
the reporter channel, both classification channels and the doublet
discriminator channel for the Luminex 100 analyzer.
The control xMAP microspheres are used to verify the calibration
and optical integrity of the system.
Software Overview
This section provides a brief overview for using the Luminex 100 IS
2.3 software.
With the Luminex 100 IS 2.3 software, you work with assay kits
provided by a kit manufacturer. A template may be included with
each kit that is imported into the software. The template includes a
sequence of commands required for the assay.
Once you select the template, you enter sample data into a “batch.”
Sample input can be done quickly with either the keyboard or a
barcode scanner. A batch can include as many samples as you have
for the assay and can include multiple microtiter plates. You can
even group multiple batches together into a multi-batch for efficient
processing. You can process a batch immediately or choose to
archive the batch for testing later.
As testing proceeds through the plate, you will see the display update
to show the wells that have been processed. Progress is shown in
both graphic and tabular form.