Luminex 100 IS Version 2.3 - Manual (Page 162)

Luminex 100 IS User Manual Version 2.3
x
MAP Technology
7 - 8 PN 89-00002-00-072 Rev. C
There is a partial clog in the
system. Clean the sample probe. See
page 6-4. Run 3 backflushes, 3
drains, 2 alcohol flushes, and 3
washes with distilled water.
There is air in the system. Run a prime and alcohol flush.
Possible problem with the laser. View the calibration trend
report. Check for dramatic
changes in temperature, sheath
pressure, or voltages. If any of
these situations are evident on
the report, Contact Technical
Support.
Zero events collected during
calibration. There is a problem with fluid
levels. Check the fluid levels in the
sheath and waste containers.
Verify that both bottles are
tightly connected to the
instrument. Check that the waste
bottle cap is vented.
Laser-related issue. Verify fluid is moving through
the system by performing a
wash. A wash function will
cause fluid to go out to waste in
five distinctive spurts. If there s
not fluid going to waste, clean
the sample probe (see page 6-4).
Run 3 backflushes, 3 drains, 2
alcohol flushes, and 3 washes
with distilled water. If the issue
does not resolve, contact
Technical Support.
Analyzer fails Controls. The control microspheres are
not fully suspended. Vortex the control vials to
resuspend the microspheres.
Wrong control lot number or
target values are entered in
Update CON Targets dialog
box. Verify that the correct lot
number and target values are
used. See page 5-46 for further
information.
The system calibrators are in the
wrong well on the plate. Verify that you placed the
control microspheres into the
correct well. See page 5-46.