Bio-Rad Image Lab™ Software - Manual (Page 105)

Displaying Gel Images
User Guide | 105
 The Low progress indicator determines which intensity value is shown at
the minimum gray scale (or other color) in the gel image.
 The Gamma progress indicator changes the gray scale curve. A value of 1
is linear. A value <1 redistributes a greater proportion of the gray scale to
the first half of the intensity values. A value >1 redistributes a greater
proportion of the gray scale to the second half of the intensity values.
You can also type numerical values in the boxes next to the progress indicators.
Clicking anywhere on the progress indicator bars moves the progress indicator
incrementally.
Options:
 Invert image display — inverts dark bands on a light background to light
bands on a dark background. Light bands on a dark background are
inverted to dark bands on a light background.
 Highlight saturated pixels — when this checkbox is selected, areas of the
image with saturated signal intensity (higher than a measurable range) are
highlighted in red.
 Linear or logarithmic histogram — this adjustment changes the y-axis on
the histogram to display the number of pixels at each intensity value using
either a linear or a logarithmic scale.
In multichannel images, you can individually highlight the saturated pixels on a
channel in red. You cannot highlight the saturated pixels in a merged image.
For Multichannel Images
In the Image Transform dialog box, you can make changes to only one image at a
time. When you work with a multichannel image, you can select each channel, in
turn, at the bottom of the Image Transform dialog box and make any changes to the
image.
In addition to the adjustments described in Image Transform on page 104 , you can
change the color of each channel. Changing the channel color in the Image
Transform dialog box automatically updates the title bar and the channel buttons on
the multichannel image.