Bio-Rad Image Lab™ Software - Manual (Page 73)

Creating a Protocol
User Guide | 73
Choose Custom to save and run an existing application with a new name or to
create and run a new application. Previously saved custom applications display
here. To create a custom application, see Setting Up a Custom Application on
page 94 .
Tip: For a list of applications with all required detection reagents, light
sources, and any conversion screens or filters noted, see on page 98 .
Note: If you select the Stain Free option, you may select the gel activation
time. See Appendix E, Using Bio-Rad’s Stain-Free Technology , on
page 247 for more information.
2. In Imaging Area, select from the list of Bio-Rad gels or enter the image area
dimensions. The red box represents the imaging area for the selected gel, and
the gray rectangle represents the imager sample stage.
3. In Image Exposure, select from one of the following options:
 Auto Exposure — this setting estimates an optimal exposure time and
ensures the best use of the dynamic range. Intense Bands optimizes for all
bands and Faint Bands makes faint bands more visible but might
overexpose more prominent bands.
 Intense Bands — optimizes exposure for all bands
 Faint Bands — a longer exposure time is used making faint bands
more visible, but more prominent bands might be overexposed