Lane and band table measurement definitions, Volume measurement definitions, Volume measurement – Bio-Rad Image Lab™ Software User Manual

Page 119

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User Guide | 119

Lane and Band Table Measurement Definitions

This section defines the measurements that display in the Lane and Band tab in the

Analysis table. Use the Data display options button to choose the columns to

display.

Band Number — each band in a lane has a unique number, sorted from top to

bottom.

Band Label — you can assign a custom label to each band by clicking the Band

Label field of the Lane and Band table.

Molecular Weight — the molecular weight of the band is calculated based on the

user-defined standard and regression method. Italic values indicate extrapolated

values. When using nucleic acid gels, the size of the band is displayed in base pairs.

Relative Front — values between 0–1 indicate the relative movement of the band

from top to bottom.

Volume — the sum of all intensities within the band boundaries.

Abs. Quant. — absolute quantification of the band.

Rel. Quant. — relative quantification of the band compared to the reference band.

Band % — percentage of the band’s volume compared to all band volumes in the

lane.

Lane % — percentage of the band’s volume compared to the entire volume of the

lane.

Volume Measurement Definitions

This section defines the measurements that display in the Volume tab in the Analysis

table. Use the Display Data Options button to choose the columns to display.

Volume Number — a unique number is assigned to each volume.

Volume Label — software-generated labels for different types of volumes

(U – unknown, B – background, S – standard). Label can be changed in Volume

Properties.

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