Bio-Rad Image Lab™ Software User Manual
Page 80
4 | Acquiring Images
80 | ChemiDoc MP Imaging System with Image Lab Software
Low Band Detection Sensitivity — this option sets detection at a low
level (25) for images with more prominent bands. Faint bands are not
detected with this setting.
High Band Detection Sensitivity — this option sets detection at a higher
level (75) for images that are fainter. Extraneous bands can be removed
later, using the Band tools in the Analysis Tool Box. See
.
Custom — select a numeric value from 1 to 100 to choose the best
detection sensitivity for your sample.
Note: You cannot specify different sensitivity levels for individual channels
of a multichannel image. The same sensitivity level is applied to all
channels. After the image is generated, you can change the sensitivity level
for individual channels using the Lane and Bands tools.
The following is a screen shot of the Detect Lanes and Bands window for
multichannel protocols.
To configure normalization settings
1.
(Multichannel protocols only) Click Normalization Channel and specify which
channel to use as the normalization channel.