Orange fluorescence reference plate – Bio-Rad Image Lab™ Software User Manual

Page 240

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D | Accessories

240 | ChemiDoc MP Imaging System with Image Lab Software

To install and use the gel alignment template kit

1.

Place the locator frame over the transilluminator with the magnetic side down.
Match the corners of the magnetic locator frame with the edges of the
transilluminator. The UV symbol on the frame will be in the same orientation as
the UV symbol on the imager.

2.

Place the gel alignment template that matches the size of your sample tray or
agarose gel into the magnetic locator frame.

3.

Place your gel or gel tray into the open area of the template.

Note: No recalibration is necessary to use the gel alignment template kit.

Orange Fluorescence Reference Plate

The orange fluorescence reference plate enables you to apply UV flat fielding

corrections to your imager. Corrections are made for all UV illumination sources,

filters, and the camera lens.

The orange fluorescence reference plate is recommended for quantitation with

Bio-Rad’s stain-free gels, and can be used to correct for image nonuniformities for

red and orange gels, including:

Ethidium bromide

GelRed

Flamingo™

Coomassie Fluor Orange

SYPRO Ruby

Krypton

Qdot 625

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