Detect the bands – Bio-Rad Image Lab™ Software User Manual
Page 148
6 | Analyzing Images
148 | ChemiDoc MP Imaging System with Image Lab Software
Detect the Bands
Use the band detection tool to detect the bands in the channels. If you are using the
total lane protein method to normalize your data, it is recommended that you do not
use the band detection tool on the normalization channel, because this will generate
large amounts of superfluous data in the analysis table. For more information, see
If you are using a housekeeping protein to calculate the normalization factor, you
must isolate this protein in your image. Remove all bands other than the
housekeeping protein from your image. The normalization channel should have only
one detected band in each lane. Use the Delete Bands tool to exclude all other
bands from the calculation.