Copying lanes, Detecting bands, Copying lanes detecting bands – Bio-Rad Image Lab™ Software User Manual

Page 138: Copying lanes on

Advertising
background image

6 | Analyzing Images

138 | ChemiDoc MP Imaging System with Image Lab Software

Copying Lanes

You can copy the lanes from one image into any other image. This tool is useful if

you want to create a multichannel image from unlinked images, and you want to

identify and match the lanes in your channels. The entire frame and all the lanes are

copied. Individual lanes cannot be copied.

To copy lanes between channels

1.

Open the Lane and Bands tool.

2.

Select the channel that contains the lanes you want to copy.

3.

From the Edit menu, select Copy.

The message Copying All Lanes appears.

4.

Select the channel that you want to copy the lanes into.

5.

From the Edit menu, select Paste.

A channel can contain only one lane frame at a time. Therefore, if you paste

lanes into a channel that already contains a lane frame, you are prompted to

confirm the deletion of the existing lanes. If you click Yes, the existing lanes are

deleted and replaced with the copied lanes.

Note: If you copy lanes into a channel of a linked multichannel image, the

lanes are copied into all channels of the image, including the Multichannel

channel.

After the lanes are copied into the channel, you can manipulate individual lanes

using the lane tools so that they are correctly positioned.

Detecting Bands

Note: Bands are detected for individual images. For multichannel images, you

must detect the bands for each channel, one at a time. This applies whether the

multichannel image is linked or unlinked. Band detection on the Multichannel

channel is disabled. The multichannel view is a composite of the other

channels. Detecting bands in this view is not particularly useful because

quantifying overlapping bands will result in combined values from multiple

channels.

Advertising
This manual is related to the following products: