Step 2. detect lanes and bands – Bio-Rad Image Lab™ Software User Manual

Page 79

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Creating a Protocol

User Guide | 79

6.

In Imaging Area, select from a list of Bio-Rad gels or enter the image
dimensions. The red line represents the imaging area for the selected gel, and the
gray rectangle represents the imager sample stage.

Step 2. Detect Lanes and Bands

To analyze the gel or blot, Image Lab must detect lanes and bands on the image.

Lanes are detected automatically, and then the background is subtracted

automatically. Refer to

Using the All Lanes and Single Lane Tools on page 134

for

details. Customize band detection with the following options.

To configure the lane and band detection settings

1.

Select the Lane and Band Detection checkbox in the left pane of the Protocol
Setup window.

2.

In the right pane, select one of the following lane and band detection options:

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