YSI 9300 Photometer - Manual (Page 131)

PHOTOMETER TEST INSTRUCTIONS
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Phenols and substituted phenols may occur in natural, drinking and industrial
waste waters. Phenols are not readily removed from water by conventional
water treatment processes. These compounds arise typically from oil and
chemical refining, livestock dips, the breakdown of pesticides, human and
animal wastes and from naturally occurring sources. Chlorination of such waters
may produce odorous and objectionable-tasting chlorophenols.
The YSI Phenoltest method provides a simple means of measuring the
concentration of phenol and phenolic compounds present in water over the range
0 - 5.0 mg/l. The concentration of phenol determined in the test is due to
unsubstituted and to ortho and meta substituted phenols. A proportion of para
substituted phenols will give a positive response.
Method
In the Phenoltest method, phenol and phenolic compounds react with 4-amino-
antipyrine in the presence of ferricyanide ions to form a red color. The reagents
are provided in tablet form and the test is carried out simply by adding the
appropriate tablets to a sample of the water. A further tablet reagent is used to
prevent interference due to metal ions.
The intensity of the red color produced in the test is proportional to the
concentration of phenolic compounds present in the sample and is measured using
a YSI Photometer.
Photometer Method
AUTOMATIC
WAVELENGTH
SELECTION
0 – 5.0 mg/l as Phenol
PHOT.54.AUTO