YSI 9300 Photometer User Manual
Page 59
 
PHOTOMETER TEST INSTRUCTIONS
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Calcium hardness is caused by the presence of calcium ions in the water.
Calcium salts can be readily precipitated from water and high levels of calcium 
hardness tend to promote scale formation in water systems. Calcium hardness is 
an important control test in industrial water systems such as boilers and steam 
raising plant; and for swimming pool waters.
The YSI Calcicol test provides a simple method of determining calcium hardness
over the range 0 - 500 mg/l CaCO
3
.
Method
The YSI Calcium Hardness test is based on the Calcicol indicator reagent method.
Calcium ions react specifically with Calcicol indicator in alkaline solution to give an 
orange coloration. The reagent itself gives a violet color in solution. Thus at 
different calcium levels a distinctive range of colors from violet to orange is 
produced. 
The reagents for the method are provided in the form of two tablets. The test is 
carried out simply by adding one of each tablet to a sample of the water. The
color produced is indicative of the calcium hardness and is measured using a
YSI Photometer.
Reagents and Equipment 
YSI Calcicol No 1 Tablets 
YSI Calcicol No 2 Tablets 
YSI 9300 or 9500 Photometer 
Round Test Tubes, 10 ml glass (PT 595)
Photometer Method
AUTOMATIC
WAVELENGTH
SELECTION
0 – 500 mg/l CaCO
3
PHOT.12.AUTO