YSI 9300 Photometer - Manual (Page 76)

Reagents and Equipment
YSI Manganese No 1 Tablets
YSI Manganese No 2 Tablets
YSI 9300 or 9500 Photometer
Round Test Tubes, 10 ml glass (PT 595)
Sample Collection
Manganese is readily absorbed onto the surfaces of sample containers. To avoid
loss of manganese test sample as soon as possible after collection.
It is important, because of the extreme sensitivity of this test, to ensure that glass-
ware used for the sample collection and test procedure is scrupulously clean. For
most accurate results in laboratory use it is recommended that all glassware is
acid-rinsed and then thoroughly washed out with deionised water before use.
Test Procedure
1 Fill test tube with sample to the 10 ml mark (see Note 1).
2 Add one Manganese No 1 tablet, crush and mix to dissolve.
3 Add one Manganese No 2 tablet, crush and mix to dissolve then cap the tube.
4 Stand for 20 minutes to allow color development (see Note 2).
5 Select Phot 20 on photometer.
6 Take photometer reading in usual manner (see photometer instructions).
7 The result is displayed as mg/l Mn.
Notes
1 Color formation is extremely sensitive to temperature. The sample temperature
should be 20° ± 1°C for optimum test results.
2 It is important to observe the standing period of 20 minutes ± 1 minute for
optimum test results. Any continuing color development or color change after
this period should be ignored.
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