Luminex xPONENT 4.0 SP1 - Manual (Page 121)

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Using the Software
• Open the Maintenance page, and then the Cmds & Routines tab. Click Warmup in
the Commands section at the left of the screen, and then click the Run button at the
bottom right to begin the procedure. The warmup process takes 30 minutes to
complete.
Calibrator xMAP beads are used to normalize the settings for the reporter channel, both
classification channels, and the doublet discriminator channel. Verification xMAP beads
are used to verify calibration and optical integrity of the system.
Once calibrated, the values remain until you recalibrate. You can track system calibration
and verification results through the Calibration report. Target value information for
calibration and verification beads is available on the Luminex website at http://
www.luminexcorp.com/support/calibration/index.html.
Calibrate your system at least once a week using the Calibration/Verification button on
the Auto Maint tab of the Maintenance page. In addition, recalibrate the system if the
delta cal temperature exceeds +/-5ºC. Verify the system daily using the Performance
Verification button on the Auto Maint tab of the Maintenance page. Refer to your assay
kit instructions for additional calibration frequency requirements.
Alternatively, you can set up the system initialization routine to include calibration and or
verification under the Admin page, System Setup tab, Maintenance Options section.
Ensure that the Luminex analyzer lasers are warmed up and the probe height is set
correctly before calibrating the system. Do not move the system waste line while
calibrating.
Run System Initialization
1. On the Home page, click System Initialization. The Auto Maint page opens.
2. Select the performance verification kit from the Performance Verification kit list at the
right of the tab.
3. Select the calibration kit from the Calibration Kit list.
4. Vortex the xMAP calibrator, verification, and fluidics containers to ensure homogeneity.
Do not dilute xMAP calibrator or verification agents.
5. Click Eject on the status bar, then place the microtiter plate on the plate holder.
6. Load a microtiter plate with at least five drops of the beads in the Calibration and
Performance Verification kit as indicated on the plate image and in the kit insert
instructions.
7. Click Retract.
8. Click Run.
Add CAL or VER Kits
Before you can calibrate the system, you will need to add xMAP calibrator and verification
bead lot information. This information is on the CD that accompanies the performance
verification kit and is also available on the Luminex website at http://
www.luminexcorp.com/support/calibration/index.html.
WARNING: Inaccurate data entry can invalidate the results of these
procedures. Careful entry of data in each field is necessary to
ensure accurate results.
1. On the Home page, click System Initialization.