Luminex xPONENT 4.0 SP1 - Manual (Page 124)

Luminex xPONENT 4.O Software Manual
112
WARNING: Human and animal samples may contain biohazardous infectious
agents. Where exposure (including aerosol) to potentially
biohazardous material exists, follow appropriate biosafety
procedures and use personal protective equipment, such as
gloves, gowns, laboratory coats, face shields, or mask and eye
protection, and ventilation devices. Observe all local, state, and
federal biohazard handling regulations when disposing of
biohazardous waste material.
When setting up a batch, if the number of samples exceeds the wells in one microtiter
plate, you can add additional plates in the Add and Change Plate secondary window.
Additional plates are identified on the bottom of the plate image as Plate a of b, where a is
the plate number and b is the total number of plates.
Create a New Batch from an Existing Protocol
Read the instructions provided with the assay kit you are using.
1. Open the Batches page.
2. Click Create a New Batch from an existing Protocol.
3. Type the batch name in the Batch Name box.
4. Type a description about the batch in the Enter Optional Description box.
5. Click a protocol you wish to use in the Select a Protocol list. If the protocol uses
standards and/or controls, view the active reagents.
6. Click Next. If the protocol uses standards and/or controls, the next tab that appears is
the Stds & Ctrls tab. Otherwise, the Plate Layout tab appears. View the details of the
active reagents or apply different assay standards/controls or manually enter new
information. Select Next.
7. On the Plate Layout tab, assign well commands for this batch. See “Plate Layout Tab”
on page 21 for a complete description of the commands and options on this tab.
8. Click Run Batch to begin batch acquisition, or click Save to save batch information to
the Pending Batch list to be run at a later time.
NOTE: If the batch spans more than one plate, the tray ejects
automatically when all defined wells have been acquired. A dialog
box displays prompting you to insert the next plate.
Create a New Batch from a New Protocol
This option creates a new batch to acquire data without using an existing protocol. It writes
raw data results to a simple ".CSV" file format. You can define parameters for samples,
gates, regions, events, on-plate and off-plate commands.
For 96-well plates, use plates with wells that will hold at least 175 µL (the extra 25 µL from
the sample, plus an extra 150 µL that are dispensed back into the well following
acquisition). For 384-well plates, use plates that will hold at least the extra volume from
the sample, plus an extra 50 µL that are dispensed back into the well following acquisition.
1. Open the Batches page.
2. Click Create a New Batch from a new Protocol to open the Settings tab.
3. Type a name in the Name box.
4. Type a description in the Description box.
5. Define the settings in the Acquisition Settings section. For information about each of
these settings, see “Settings Tab” on page 24.