Delete batch, Edit batch, Run batch – Luminex xPONENT 4.0 SP1 User Manual
Page 127: Performing analysis
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Using the Software
Delete Batch
You can only delete unprocessed batches. Batches are deleted from the Open Batch list
and moved to the Open Incomplete Batch list.
1. Open the Batches page.
2. In the Pending Batches section, click the batch you want to delete, then click Delete.
The Delete Pending Batch dialog box opens.
3. Click Yes.
Edit Batch
1. Open the Batches page.
2. Click the batch you want to edit, then click Edit. The Protocol tab opens.
3. Edit the information as needed on the Protocol, Std & Ctrls, and Plate Layout tabs.
4. Click Save on the Plate Layout tab.
See “Settings Tab” on page 24 for more information on batch settings.
NOTE:
Run Batch
Open the Batches page. Select the pending batch that you want to run, then click Run.
NOTE:
If the batch spans more than one plate, the tray ejects
automatically when all defined wells have been acquired. A dialog
box displays prompting you to insert the next plate.
Performing Analysis
If you are using a third-party software to perform analysis, please see the user manual
provided with that software.
You may direct the system to acquire samples in replicate regardless of batch type. For
qualitative batches, qualitative results for replicates are averaged and the reported
interpretation is determined from this replicate average.
Replicates in quantitative batches are based on a standard curve that is generated by
either the “Fit of all standards” or “Mean of replicates”. The default is “Fit of all standards”.
Unknown samples are calculated from the standard curve. Test results for replicate
samples are averaged to determine the reported quantitative result denoted as “AVG”.
You can analyze an acquired batch using the analysis features of Qualitative,
Quantitative, and Allele Call algorithms.
Qualitative Analysis. Determines results as either positive or negative, reactive or non-
reactive, and so on. The system is flexible in defining custom result ranges, such as
negative, low positive, and high positive.
Quantitative Analysis. Determines the sample concentrations from standard curves
using regression methods, such as 4P or 5P logistic curve fitting.