Luminex xPONENT 4.0 SP1 - Manual (Page 147)

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Glossary
Qual A value that determines a cutoff or threshold. This, in conjunction with
ranges using the Lum Qual Formula, Adv Qual Formula, or an edited
range specific for your assay, helps to determine qualitative results for
unknown samples.
qualitative Pertaining to calculations that determine the absence or presence of
an analyte.
quantitative Pertaining to calculations that determine the precise numerical
measurement of an analyte.
reagent A substance used to detect or measure other substances.
region event A gated event that exhibits the correct classification fluorescence
values to fall into one of the bead regions. Region events are used to
calculate the median fluorescence intensity (MFI) which is the final
data generated by the system. Any other event is excluded from data
analysis.
reporter A molecule (or combination of molecules) with a specific range of
excitation and emission wavelengths that is used to identify or quantify
an analyte. Examples of acceptable reporters are Phycoerythrin and
Alexa 532. Reporter signals are the final result of each assay.
reporter channel (RP or RP1) A specific range of wavelengths that includes the emission wavelength
of a designated reporter molecule.
RP1 Refers to the dyes bound to the surface of the xMAP microsphere. Also
see reporter channel.
RP See reporter channel.
sample The mixture of assay components (microspheres, reporter, patient
dilutant) that are analyzed.
sample probe The probe, or needle, on the Luminex analyzer that acquires sample
from the 96-well plate.
sample reaction The reaction that occurs between reagents and beads.
sheath fluid Luminex xMAP Sheath Fluid is the delivery medium of the sample to
the optics component. Only Luminex-approved sheath fluid should be
used when operating the Luminex analyzer.
signal Detectable measurement unit of the reporter molecule.
standards microspheres, assay Assay standards are substances of known concentrations used to
derive a standard curve with which unknown samples and controls are
compared to determine their concentration or quantity. See control