Luminex xPONENT 4.0 SP1 - Manual (Page 75)

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Touring the Software
Command Sequence. Contains the command sequence for the active plate. The list
includes all active wells, the type of command (Unknown, Standard, Control, Background,
or assigned maintenance command), ID, and dilution factor. Double click the ID field to
type an ID. Double click the Dilution field to type a dilution factor.
A command’s ID and Dilution fields have a blue border around them if they can be double
clicked to type information.
Move Command. These arrows move a selected command up or down in the Command
Sequence list, changing the acquisition order.
Import List. Opens the Open dialog box to import an existing command sequence list.
Replicate Count. Defines a quantity of replicate sets from one to nine.
Grouping. Selects the sequence in which the replicates are laid out in plate wells. The
options are:
• 123123123. . . Lays out one of each replicate set at a time in numerical order.
• 111222333. . . Lays out all the replicates in a set before moving on to the next set in
numerical order.
You can assign the following well commands. Each command is associated with a color.
You can click and drag to highlight a series of wells, click a column or row header to
highlight the entire column or row, or simply click and highlight different wells and then
click a command below to assign that command to all the highlighted wells.
• Unknown (U): Yellow
• Background (B): Purple
• Control (C): Red
• Standard (S): Green
• Wash (W): Blue
The Delete and Start at Well commands are also available to assign as well commands.
Delete removes the well command for the selected well. The Start at Well command
enables you to begin acquisition at a well other than A1.
NOTE: You should first delete all standards from the plate layout if any of
the standards need to be rearranged, and all controls from the
plate layout if any of the controls need to be rearranged.
NOTE: Wells and commands you assign to the protocol plate layout are
saved into the protocol settings and execute each time you use
the protocol to run a batch. Standards and controls associated
with a given protocol typically remain constant, while the number
of unknown wells often vary. You can assign a specific number of
unknown wells to the plate when setting up a batch.
Commands:. Assigns maintenance commands that run before or after single wells,
ranges of wells, and rows or columns of wells. Select a well, and then select one of the
following commands:
• Alcohol Flush
• Prime
• Sanitize