Edit quantitative analysis settings – Luminex xPONENT 4.0 SP1 User Manual

Page 134

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Luminex xPONENT 4.O Software Manual

122

WeightType list. Click Apply to All Analytes to apply the selection to all analytes. To
edit an analysis setting, see “Edit Quantitative Analysis Settings” on page 122.

15.To change the analysis for a single analyte, click the Analysis field for the analyte you

wish to modify. The Analysis Settings dialog box opens.

16.Select an analysis method in the Method list.
17.Select a weight type in the WeightType list (weight type may not display, depending on

the analysis method selected in the Method list).

18.Check Mark as Intra-Well Normalization Bead if you wish to use the analyte as a

normalization bead.

19.Check Use Threshold Ranges if you wish to use a range for the analysis.
20.Click Add Range to setup the threshold range.
21.Type a name for the range in the Range Name field.
22.Type low and high range values in the Low Value and High Value fields.
23.Select the check box in the Inclusive column to include the value in the range, or leave

it clear to make the range value one unit higher than the low value, and one unit lower
than the high value.

24.Highlight a range and click Delete Range to delete the range.
25.Click OK to apply the new settings to the analyte first clicked, or Apply to All Analytes

to apply them to all the analytes in the protocol.

26.Click Next. The Plate Layout tab opens.

To add well commands, highlight the appropriate wells and mark them as unknown,
standard, control, background, or wash. You can also delete commands that you’ve

added, and change the starting location on the plate. If you wish to run in replicate,

change the Replicate Count to the appropriate number and the Grouping to your

preferred grouping method.

To add maintenance commands, choose the desired command from the Command
list. Highlight the well that you want to apply it to, and then choose Before Well

Command or After Well Command. If you are working with more than one plate, click

Add Plate to select plates to display in the Plate list.

Delete a command by clicking the well, and clicking Delete. The Delete Options
dialog box opens. Select Delete just the selected wells to delete a single well

command, or Delete all wells containing these samples to delete all wells with the

same command.

As you add commands to your plate, they appear in the Command Sequence list.
Type a well ID in the ID field. Here, you can give each of your wells an ID. You can also

import a protocol file by clicking Import List.

Move commands up or down in the sequence by highlighting a command and using
the Move Command arrows to move the command up or down in the list.

27.Click Save.

For more information about the commands and settings on the Plate Layout tab, see
“Plate Layout Tab” on page 62.

Edit Quantitative Analysis Settings

You can only edit Luminex 4 Parameter Logistic and Luminex 5 Parameter Logistic
analysis methods.

1. Open the Protocols page, then open the Protocols tab. Select a protocol and click

Edit.

2. From the Analytes tab, click an analyte’s Analysis field to open the Analysis

Settings dialog box.

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