Luminex xPONENT 4.0 SP1 - Manual (Page 135)

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Using the Software
3. From the Method list, click No Analysis, Cubic Spline, Linear Fit, Logistic 4P, or
Logistic 5P.
4. From the Weight list, click None or 1/Y
^2
.
NOTE: The Weight options are only available if the chosen Method is
Logistic 4P or Logistic 5P.
5. Check Mark as Intra-Well Normalization Bead if you wish to use a normalization
bead. The normalization bead is a microsphere set that is included in the assay as an
internal control. It controls for sample variation and can be used to normalize data
between samples in a run.
6. Check Use Threshold Ranges if you wish to use a range for the analysis.
7. Click Add Range to setup the threshold range.
8. Type a name for the range in the Range Name field.
9. Type low and high range values in the Low Value and High Value fields.
10.Select the check box in the Inclusive column to include the value in the range, or leave
it clear to make the range value one unit higher than the low value, and one unit lower
than the high value.
11.Highlight a range and click Delete Range to delete the range.
12.Click OK to apply the new settings to the analyte first clicked, or Apply to All Analytes
to apply them to all the analytes in the protocol.
Create an Allele Call Protocol
This protocol contains no standards or controls. An allele call compares groups of two,
three, or four analytes to identify genotypes.
1. Open the Protocols page, then open the Protocols tab. Click Create New Protocol.
The Protocol Settings tab opens.
2. In the Name box, type the name of the protocol.
3. In the Description box, type a description of the protocol.
4. In the Version box, type the version of the protocol.
5. In the Manufacturer box, type the manufacturer information for the protocol.
6. Define the settings in the Acquisition Settings section. For information about each of
these settings, see “Acquisition Settings” on page 57.
7. Define settings in the Analysis Settings section, selecting Allele Call as the analysis
type. For information about the settings in this section, see “Analysis Settings” on
page 58.
8. Click Next. The Analytes tab opens.
9. Click the desired analyte (bead ID) in the numbered grid. Information about the analyte
appears in a list on the right side of the grid. For an allele call analysis, you must select
a group of two, three, or four analytes.
10.Click Group to group the analytes for the allele call. The grouped analytes display in a
list to the right. Select more analytes and then Group again if you wish to add another
group to the analysis.
11.Click and type an analyte name in the Name column to the right of the analyte grid.
12.In the Count box, type the total desired bead count for each analyte. Click Apply All.
13.To set a bead count and the units for a single analyte, click in the Units and Count
columns directly to the right of the analyte grid, and type a units value and bead count.
14.Click in the Call % column and type a value to set an individual analyte’s call
percentage.
15.Click Next. The Plate Layout tab opens.