Luminex xPONENT 4.0 SP1 User Manual

Page 52

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Luminex xPONENT 4.0 Software Manual

40

NOTE:

You can change the X axis of the histogram for troubleshooting
purposes. Use only the default settings in all other instances.

Gate - Show, create, delete, or move the gate. You can also move the gates by clicking
and dragging the red lines on the histogram.

Autoscale - Click to automatically adjust the maximum number of events shown on the
Y axis. Click during acquisition to readjust the Y axis scale.

Set Scale - The Set Scale dialog box displays. Set the maximum number of events
and click OK. The default number of events is 60.

View - You can choose to view all events, or only gated events.

Display Mode - Opens the Histogram Options dialog box. Click either Logarithmic
or Linear display. Logarithmic display is the default. Select the Zoom check box for a

magnified view of the histogram.

Dot Plot. The default location of the dot plot is the lower-right section of the Current
Batch
tab. The dot plot is a graphical display of real-time data collection. Luminex
recommends using the default settings to collect data.

Right-click inside the dot plot area to access the following options:

X Axis - Changes the X axis to the following: Doublet Discriminator, Reporter 1,
Classification 1, Classification 2, and Classification 3.

NOTE:

You can change the X axis of the dot plot for troubleshooting
purposes. Use only the default settings in all other instances.

Y Axis - Changes the Y axis to the following: Doublet Discriminator, Reporter 1,
Classification 1, Classification 2, and Classification 3

View - Choose to view all events or only gated events.

Display Mode - Select either Logarithmic or Linear display. The software defaults to
Logarithmic display. Click maximize for a magnified view of the dot plot.

Plot Type - Select either Density Plot or Decaying Plot. The software defaults to
Density Plot, which displays a constant accumulation of events. Contrasting colors

indicate increasing density. The Decaying Plot plots only the last 100 events.

NOTE:

The Density Plot allows the visual elimination of data values that
are determined to be insignificant to the display. Luminex
recommends collecting data in Density Plot mode to observe all
collected events. You can only view the Decaying Plot during
batch acquisition.

Right-click inside the dot plot area to access the following options:

Log. Displays a log of system processes. The log includes the following information:

Date

Message

Code

Log entries indicating warnings are highlighted yellow. Errors are highlighted in red. Other
log entries are not highlighted. Click the maximize button to maximize the log. The log
expands to fill the whole window. Click minimize to return to the standard size.

Save Image. Opens a Save As dialog box to save a screen capture.

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