Luminex xPONENT 4.0 SP1 User Manual

Page 70

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Luminex xPONENT 4.0 Software Manual

58

Analysis Settings. Use this section to set the analysis type, set the number of standards
and controls, select an external analysis program, and choose whether to analyze results
while acquiring samples.

Analysis Type - Use this list to choose from the following analysis types:

None - No analysis. Select if you have your own data post-processing program and
want to obtain only fluorescent intensity results. You cannot apply standards or
controls when you select None. You cannot analyze acquisitions with this setting.

Qualitative - Qualitative analysis determines results as either positive or negative,
reactive or non-reactive. The software is flexible in defining custom result ranges,
such as negative, low positive, or high positive. Determinations are based on a
single standard. For qualitative analysis the Luminex software uses a specific
algorithm, shown below.

Where FI = Fluorescent Intensity and Ki = a “Quali” value entered in lot information to
determine the value or the qualitative assay standard.

The “Quali” value determines a cutoff or threshold. This, in conjunction with ranges
using the Lum Qual formula or an edited range specific for your assay, helps to
determine qualitative results for unknown samples.
Two predefined formulas using the algorithm are included in the system. You can use
them as is or edit their range values to meet your requirements. For information about
changing the analysis settings, see “Analysis Settings Dialog Box” on page 28 and
“Analysis Settings Dialog Box” on page 29.

Quantitative

-

Determines the sample concentrations from standard curves using

regression methods Cubic Spline, Linear, Logistic 4P,

and

Logistic 5P. Type the

desired values for standards and controls in the Number of Standards and Number
of Controls
boxes. Select either Fit of All Standards or Mean of Replicates for the
calculation of the curve fit.

NOTE:

Luminex recommends Fit of All Standards as the most accurate
calculation of the curve fit.

Allele Call

-

Sets analysis for an allele call. Analytes must put in groups of 2, 3, or 4

analytes

.

Min MFI Enabled - Select this box to enable a minimum MFI for the Allele Call
analysis. Type a value in this box to set the minimum MFI for analysis.

Number of Standards - Click to type the number of standards for the protocol.

Number of Controls - Click to type the number of controls for the protocol.

Fit of all Standards - The standard curve will be determined by using each individual
standard replicate when calculating the standard curve. For example if one runs

duplicates of a 7 point standard curve, the software will calculate the standard curve by

using 14 points.

Mean of Replicates - The standard curve will be determined by averaging the
individual standard replicates when calculating the standard curve. For example if one

runs duplicates of a 7-point standard curve, the software will calculate the standard

curve by using 7 averaged points.

Analyze Results While Acquiring Samples - The software allows for real-time
viewing of the results as the instrument analyzes samples.

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