Leica Biosystems CM1860/CM1860 UV - Manual (Page 14)

14 Instructions for Use V1.4 – 10/2012
2.5 Operating the instrument
2. Safety
2.6 Cleaning and disinfection
• Take care when handling microtome knives and disposable blades. The cutting edge is
extremely sharp and can cause serious injuries!
We strongly recommend using the safety gloves included with the standard delivery!
• Never leave knives and knife/blade holders with a knife/blade mounted lying around!
• Never place a knife on a table with the cutting edge facing upward!
• Never try to catch a falling knife!
• Always clamp the specimen first, then the knife.
• Before handling the specimen or the knife, changing the specimen or taking a break, lock
the handwheel and ensure that the knife is covered by the knife guard.
• Extended skin contact with the cold parts of the instrument may cause frostbite!
• To make sure that the condensation water stemming from the defrost cycles drains into the waste
container and to avoid the risk of possible contamination, ensure that the tap of the waste container
(2, Fig. 54 ) is open whenever operating the cryostat. Only shut the tap when draining the waste
container!
The use of flammable sprays within the cryostat chamber is permitted only with the Leica
Cryofect tested by us.
At cryochamber temperatures of 0 °C to -35 °C! Spray a MAXIMUM of 10 sprays into the middle
of the cryostat chamber and close the sliding glass door. After each disinfection, the chamber
must be wiped carefully with a cloth and briefly ventilated!
* Cryofect is not sold in all parts of the world
Remove section waste after EVERY change of specimen. Do not start disinfection until you
have done this! Each new specimen is a potential source of contamination.
We strongly recommend defrosting the CM1860/CM1860UV Cryostat completely for 24 hours,
1-2x annually or as needed.
• It is not necessary to remove the microtome for routine disinfection. The instrument has
been designed for UV disinfection. Spray disinfection with Leica Cryofect* is also possible,
thanks to the special insulation of the microtome.