5 defrosting, 1 automatic defrosting of the cryochamber, 2 manual defrosting of the cryochamber – Leica Biosystems CM1860/CM1860 UV User Manual

Page 52: Daily use of the instrument

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52

Instructions for Use V1.4 – 10/2012

7.5 Defrosting

Defrosting the cryochamber actually means defrosting the evaporator to

prevent excessive frost buildup. The evaporator is flushed with hot gas

during defrosting. The cryochamber virtually is frostfree and does not need

to be defrosted.

The condensation water that is produced during defrosting is collected in a

container, which is located at the front of the cryostat cabinet.

To make sure that the condensation water stemming from the de-

frost cycles drains into the waste container and to avoid the risk of

possible contamination, ensure that the tap of the waste container

(2,

Fig. 54

) is open whenever operating the cryostat.

Only shut the tap when draining the waste container!

The quick freeze shelf is cooled during the automatic cryochamber

defrosting. However, the Peltier element is turned off.

The maximum duration of a defrost cycle is 12 minutes. Defrosting

is terminated automatically once the cryochamber has reached a

temperature of -5 °C. Cooling turns back on automatically.

7.5.1 Automatic defrosting of the cryochamber

An automatic defrost cycle takes place once in 24 hours.
You can program the time of the automatic defrost cycle on control panel

field 1 (

see item 6.2.2

).

7.5.2 Manual defrosting of the cryochamber

To avoid an unintended defrosting, the activation of a manual de-

frost cycle is confirmed by an audible signal.

Cooling turns back on automatically.

In addition to the programmed automatic defrost, a manual defrost for the

cryochamber can also be activated (

see item 6.2.6

).

7.

Daily Use of the Instrument

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