5
Leica CM1860/CM1860UV – Cryostat
7.
Daily Use of the Instrument .................................................................................................................. 35
7.1
Specimen freezing ..................................................................................................................................... 35
7.1.1 Quick freeze shelf ....................................................................................................................................... 35
7.1.2 Stationary heat extractor
(optional) ............................................................................................ 36
7.2
Specimen discs .......................................................................................................................................... 36
7.2.2 Specimen orientation ................................................................................................................................ 36
7.2.1 Inserting the specimen discs into the specimen head ........................................................................ 36
7.3 Sectioning .................................................................................................................................................... 37
7.3.1 Blade holder CE .......................................................................................................................................... 37
7.3.2 Blade holder CE-TC .................................................................................................................................... 43
7.3.3 Knife holder CN ........................................................................................................................................... 44
7.3.4 Knife holder CN-Z with anti-roll guide .................................................................................................... 48
7.3.5 Cleaning the blade and knife holders .................................................................................................... 48
7.3.6 Installing the shelf (movable) (optional) ................................................................................................. 50
7.4
Temperature selection chart (in minus °C) ............................................................................................ 51
7.5 Defrosting .................................................................................................................................................... 52
7.5.1 Automatic defrosting of the cryochamber ............................................................................................. 52
7.5.2 Manual defrosting of the cryochamber .................................................................................................. 52
7.5.3 Manual defrosting of the quick freeze shelf .......................................................................................... 53
7.6
Terminating work ....................................................................................................................................... 53
7.6.1 Terminating daily work .............................................................................................................................. 53
7.6.2 Shutdown for a longer period .................................................................................................................. 54
8. Troubleshooting ..................................................................................................................................... 55
8.1
Error messages in the display .................................................................................................................. 55
8.2
Temperature control button ..................................................................................................................... 56
8.3
Possible sources of error, causes and remedies ................................................................................. 57
8.3.1 Changing the battery ................................................................................................................................. 61
9.
Cleaning, Disinfection, Maintenance ................................................................................................ 62
9.1 Cleaning ....................................................................................................................................................... 62
9.2
Spray disinfection with Leica Cryofect ................................................................................................... 63
9.3 Maintenance ............................................................................................................................................... 64
9.3.1 General maintenance instructions .......................................................................................................... 64
9.3.2 Replacing the fuses ................................................................................................................................... 66
9.3.3 Replacing the UVC lamp ............................................................................................................................ 66
9.3.4 Replacing the fluorescent lamp ............................................................................................................... 68
10.
Ordering Information, Components and Consumables ................................................................... 69
10.1 Ordering information .................................................................................................................................. 69
10.2 Mobile heat extractor –
application ............................................................................................ 94
10.3 Cold extractor –
application ........................................................................................................ 94
11.
Warranty and Service ........................................................................................................................... 95
12.
Decontamination Certificate (Master) ............................................................................................... 96
