Leica Biosystems ASP200 S - Manual (Page 22)

22 Instructions for Use V 1.4, RevE – 03/2013
3. Instrument Components and Specifications
The following reagents may be used in the Leica ASP200 S:
Fixatives Dehydration Clearing Paraffinizing
1st Formalin
(buffered or
unbuffered)
1st Ethanol 1st Xylene 1st Wax
2nd Isopropanol 2nd Toluene*
3. Methanol 3. Chloroform*
2nd Formalin re-
placement
4. Butyl alcohol
5. Industrial methyl-
ated spirits
*
Before using these reagents, please obtain information from Leica or the supplier about required
preventive measures.
Reagents other than those listed here may damage some components of the instrument. Do not
use acetone, benzene or trichlorethane in the instrument.
Fixatives containing mercuric salts, acetic or picric acid will corrode metallic components in
the instrument and shorten instrument life.
If you choose to work with such fixatives, it is essential to perform a clean cycle which con-
tains multiple water rinses each time after use, to minimize damage.
In addition, we recommend frequent and regular preventive maintenance by the Leica Techni-
cal Service.
3.5 Compatible reagents (continued)
3.6 Recommended reagent handling
• The reagents used should be replaced after 600 to 1200 specimens have been processed or
after 6 cycles of 100 to 200 specimens each.
• For formalin, process alcohol and process xylene reagents, ambient temperature is recom-
mended.
• The recommended temperature for cleaning reagents in the cleaning cycle is 65 °C.
• Only zinc formalin based on zinc sulfates may be used in the ASP200 S/ASP300 S. If zinc for-
malin is used, the cleaning program must include an additional cleaning water step.
• The use of formalin reagents containing zinc chloride can cause corrosion in and on the
instrument.