Luminex IS Version 2.3 - Manual (Page 105)

PN 89-00002-00-110 Rev. B 95
x
MAP Technology Luminex xMAP microsphere set
Luminex xMAP
microsphere set
Luminex multi-analyte microspheres containing a unique mixture of
two distinctly colored fluorochromes to distinguish them from other
multi-analyte microspheres.
laser
Light Amplification by Stimulated Emission of Radiation (laser).
This highly purified source of light is an efficient way to excite
fluorochrome electrons.
microparticle
A solid substance with a diameter in the micrometer range. Often
used as a synonym for a microsphere.
microspheres
Polystyrene spheres with a diameter in the micrometer range. Also
called beads.
multi-analyte
Several assays or tests performed simultaneously in the same
reaction container.
multi-batch
A set of batches to be processed consecutively.
photobleaching
The process in which light absorption converts the fluorochromes
inside the beads into different fluorescent or nonfluorescent
compounds. Photobleaching prevents beads from being properly
classified.
PMT
Photomultiplier tube, measures the excitation emission intensity of
the reporter dye bound to the surface of the xMAP microspheres.
product
A group of standards and controls that you use to create a template.
Products are analogous to “reagent kits”. They are used in the
template’s definition for the multi-analyte assays that will be
performed.
Quali
A value that determines a cutoff or threshold. This in, conjunction
with ranges using the Lum Qual Formula, Adv Qual Formula, or an
edited range specific for your assay, helps to determine qualitative
results for unknown samples.
qualitative
Pertaining to calculations that determine the absence or presence of
an analyte.
quantitative
Pertaining to calculations that determine the precise numerical
measurement of an analyte.
reporter
A molecule (or combination of molecules) with a specific range of
excitation and emission wavelengths that is used to identify or