Luminex IS Version 2.3 User Manual

Page 24

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Luminex IS Software Manual for Version 2.3

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MAP Technology

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PN 89-00002-00-110 Rev. B

Figure 12 Acquisition Detail Toolbar

The Histogram, in the lower left section of the Acquisition Detail
tab, defaults to display the Doublet Discriminator (DD) on the X axis
and Events on the Y axis. Doublets appear when two microspheres
stick together, creating undesired results. When you enable the gate,
two vertical red, dashed lines appear to represent gate positions
determined by the template or settings established within the New
Advanced Batch. After setting the gate, everything outside the gate is
ignored. You cannot change the gate position during batch
acquisition. You can change the gate positions before data
acquisition, after the system finishes acquiring data, or after pausing
the system. The change is visual. The data is still collected according
to the gate positions set in the assay template or New Advanced
Batch setup. The gate in effect when the system collects data
determines which values to use to obtain the result. Applying a gate
or changing a gate for existing data does not change your calculated
values. The gate positions, also located in the lower corner of the
histogram, are used while collecting data are the numerical values
selected in the template or the New Advanced Batch.

1. Gate Boundaries 2. Aggregate Beads 3. Numerical Gate Position

Figure 13 Set DD Gate Example

1. Replay Batch

6. Cancel All

2. New Advanced Batch

7. Eject/Retract

3. View Batch Data

8. Pause

4. Start Plate

9. Resume

5. Cancel Command

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