Luminex IS Version 2.3 User Manual

Page 106

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96

PN 89-00002-00-110 Rev. B

Luminex IS Software Manual For Version 2.3

x

MAP Technology

quantify an analyte. Examples of acceptable reporters are
Phycoerythrin and Alexa 532.

reporter channel (RP or RP1)

A specific range of wavelengths that includes the emission
wavelength of a designated reporter molecule.

RP1

Refers to the dyes bound to the surface of the xMAP microsphere.
Also see reporter channel.

RP

See reporter channel.

sample

The mixture of assay components (microspheres, reporter, patient
diluent) that are analyzed.

sample reaction

The reaction that occurs between your reagents and the beads.

sheath fluid

Luminex xMAP Sheath Fluid is the delivery medium of the sample
to the optics component. Only Luminex- approved sheath fluid
should be used when operating the Luminex analyzer.

signal

Detectable measurement unit of the reporter molecule.

standards microspheres,
assay

Assay standards are substances of know concentrations used to
derive a standard curve with which unknown samples and controls
are compared to determine their concentration or quantity. See
control microspheres, assay.

suspension

Solution consisting of homogeneously dispersed microspheres in an
aqueous medium.

system controls

Include the xMAP reporter and classification control microspheres.
They are used to verify the calibration of the Luminex 200 analyzer.

template

A sequence of commands and predetermined settings defined by the
kit manufacturer.

test

Each test represents an analyte and corresponds to a bead set which
is represented by a bead ID.

verification

The process using system controls to ensure the analyzer is
functioning properly with current calibration settings.

xMAP

See Luminex xMAP microsphere set.

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