Bio-Rad Microplate Manager Software - Manual (Page 105)

Chapter 6. Data Formatting and Reports
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defined, they are plotted together with their respective regression curves (although
only the external standards are used to calculate unknown concentrations).
In calculating the curve, you can select among five regression methods: Linear,
quadratic, cubic, cubic spline, and logistic. To change the regression method, click
on the appropriate button at the bottom of the dialog box, or select the desired
method under the OPTIONS menu.
½
For more information about standard curve fitting, see section 6.8 below.
You can also change the axis scale among four different combinations of linear and
logarithmic (linear-linear, log-linear, linear-log, and log-log) for every regression
method except logistic; the logistic curve fit is constrained to linear-linear axes. To
change the axis scale, click on the appropriate button at the bottom of the dialog box,
or select the desired scale under the OPTIONS menu.
½
If you are plotting negative kinetic velocities, then the velocity or maximum
velocity axis of the Standard Curve Report is constrained to linear unless the
Negative Kinetics box is checked (logarithms cannot be taken of negative
values). See section 5.3.2.
When the mouse is moved within the graph area, the coordinates of the pointer
appear in the status bar at the bottom of the Microplate Manager window. (The status
bar display is toggled on and off by selecting Status Bar under the VIEW menu).