Bio-Rad Microplate Manager Software - Manual (Page 14)

Microplate Manager User Guide
4
Data Files
The data file contains the raw data from the microplate reader. It has the extension
.mpm.
For an endpoint reading, this data consists of the individual absorbance readings
from the wells on the microplate. For a kinetic reading, this data is the time course
series of the individual absorbance readings. A multiple-plate reading creates a new
data file for each plate; each file contains endpoint absorbance measurements.
The data file also contains a plate template, which contains all the information
necessary to analyze the absorbance data, including a description of the assay, well
types, concentrations, dilutions, and the regression method used for computing
unknowns from standards. When the data file is first created after the absorbance data
is collected, the template associated with the protocol file is copied into the data file.
You can later edit the template or completely replace it with a template from another
data file.
Finally, the data file includes some information on how the plate was read (e.g.,
measurement wavelength, date/time of reading).
1.3 New Features in Version 5.0
This version of Microplate Manager features an enhanced user interface with image
display and grid overlay features. Some of the new features of the software include:
•
Flexible plate definition to handle images reads of plates from 1 to 1,536 wells,
including drag-and-drop well layout and multilevel undo when formatting
images.
•
Improvements in template formatting, including automatic scrolling when
formatting large plates, noncontiguous well selection for changing assay and
replicate set association, better handling of renumbering standards and unknowns
on densely formatted plates, and autofilling for rapidly assigning standard and
unknown replicate sets.
•
Automated setup. The software automatically identifies the microplate reader
and establishes the connection to the reader.