Bio-Rad Microplate Manager Software - Manual (Page 63)

Chapter 5. Protocols
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When a template is saved in a protocol file, it will remain there until you select a
different template.
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If you create or edit a template in one protocol and want to copy it to another
protocol, you must first save it as a data file. To do so, you have to click on Run.
This will create a data file with a copy of the template in it. You can then save
this data file and open it from within the new protocol.
5.1.3 Setting the Reading Parameters
Microplate Manager will read a plate in either a single-wavelength or a dual-
wavelength mode. To select the filter wavelength (in nanometers) for measurement
and reference readings, go to the Reading Parameters section of the protocol dialog
box.
Figure 35. Reading Parameters in the protocol dialog box.
The wavelengths you have specified for your microplate reader’s filters are listed in
the pulldown menus. See section 2.7 for instructions on how to set the filter
wavelengths for your microplate reader.
If you select the Single button, one measurement filter will be used for your reading.
If you select the Dual option, you can also select a reference filter. The reference
filter will not capture any data, but will provide a baseline reading that you can
subtract from your measurement reading.
5.1.4 Incubator Controls (Ultramark and Benchmark only)
To use the incubator of the Ultramark or Benchmark, click on the Incubator On
checkbox in the protocol dialog box.